4B49
1.15 A Structure of Lysozyme Crystallized without 2-methyl-2,4- pentanediol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Collection date | 2011-04-23 |
| Detector | ADSC |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 76.841, 76.841, 38.691 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.670 - 1.150 |
| R-factor | 0.1279 |
| Rwork | 0.127 |
| R-free | 0.15053 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | INHOUSE MODEL |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.349 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.170 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.080 | 0.760 |
| Number of reflections | 41718 | |
| <I/σ(I)> | 41.5 | 3.2 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 13.5 | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | CRYSTALLIZATION BY HANGING DROP AT 4C. CONDITIONS IN DROP: PROTEIN AT 35 MG/ML IN 200MM TRIS PH 8.0. CONDITIONS IN RESERVOIR: 200MM TRIS PH 8.0. |
