4B44

Bacterial translation initiation factor IF2 (1-363), complex with GDP at pH8.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	295
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	45.150, 62.200, 159.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.452 - 2.700
R-factor	0.2175
Rwork	0.212
R-free	0.27130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4b3x
RMSD bond length	0.014
RMSD bond angle	1.212
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE: DEV_1269))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.160	1.130
Number of reflections	12840
<I/σ(I)>	11.5	1.8
Completeness [%]	99.0	99.5
Redundancy	4.3	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		pH 8.0