4B41
Crystal structure of an amyloid-beta binding single chain antibody G7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2010-08-19 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.178, 32.596, 52.197 |
| Unit cell angles | 103.74, 99.54, 90.62 |
Refinement procedure
| Resolution | 49.936 - 1.191 |
| R-factor | 0.1374 |
| Rwork | 0.136 |
| R-free | 0.16190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ezj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.132 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.940 | 1.260 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmerge | 0.030 | 0.150 |
| Number of reflections | 50156 | |
| <I/σ(I)> | 18.6 | 6.7 |
| Completeness [%] | 85.1 | 62.6 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.2 | 0.1 M NA CITRATE PH 5.2, 0.2 NACL, 26-30% PEG8K |
