4B3T
Crystal structure of the 30S ribosome in complex with compound 39
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-06 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 401.730, 401.730, 173.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.000 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.24340 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1j5e |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.222 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.120 | 1.000 |
| Number of reflections | 280162 | |
| <I/σ(I)> | 12.1 | 2.2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 7.4 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 4% MPD, 75MM NH4CL, 200MM KCL, 15MM MAGNESIUM CHLORIDE, MES-KOH PH 6.5,VAPOR DIFFUSION, HANGING DROP AT 277K |
