4B3G

crystal structure of Ighmbp2 helicase in complex with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF
Synchrotron site	ESRF
Temperature [K]	100
Spacegroup name	P 31 2 1
Unit cell lengths	87.288, 87.288, 372.692
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.970 - 2.850
R-factor	0.2108
Rwork	0.206
R-free	0.29220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.338
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.970	2.850
High resolution limit [Å]	2.850	2.850
Rmerge	0.030	0.550
Number of reflections	39419
<I/σ(I)>	12.5	1.9
Completeness [%]	90.2	99.5
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1