4B33
Humanised monomeric RadA in complex with napht-2-ol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-10 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.660, 78.710, 39.670 |
Unit cell angles | 90.00, 117.91, 90.00 |
Refinement procedure
Resolution | 33.073 - 1.499 |
R-factor | 0.1901 |
Rwork | 0.188 |
R-free | 0.22690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1PZN CHAIN A |
RMSD bond length | 0.006 |
RMSD bond angle | 1.568 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.590 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.040 | 0.400 |
Number of reflections | 31815 | |
<I/σ(I)> | 19.18 | 3.04 |
Completeness [%] | 96.7 | 95.2 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.2 | 8% PEG-1000, 100 MM NAK PHOSPHATE, PH 6.2 |