4B32
Humanised monomeric RadA in complex with napht-1-ol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.690, 79.050, 39.620 |
| Unit cell angles | 90.00, 117.88, 90.00 |
Refinement procedure
| Resolution | 33.064 - 1.500 |
| R-factor | 0.1911 |
| Rwork | 0.189 |
| R-free | 0.22760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1PZN CHAIN A |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.577 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.530 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 0.380 |
| Number of reflections | 32695 | |
| <I/σ(I)> | 18.36 | 3.4 |
| Completeness [%] | 99.2 | 98.8 |
| Redundancy | 3.7 | 3.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 8% PEG-1000, 100 MM NAK PHOSPHATE, PH 6.2 |
