4B2L
Humanised monomeric RadA in complex with L-methylester tryptophan
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-10 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.620, 78.770, 39.630 |
Unit cell angles | 90.00, 118.06, 90.00 |
Refinement procedure
Resolution | 39.310 - 1.500 |
R-factor | 0.1881 |
Rwork | 0.186 |
R-free | 0.22300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1PZN CHAIN A |
RMSD bond length | 0.006 |
RMSD bond angle | 1.545 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.090 | 1.590 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.040 | 0.470 |
Number of reflections | 31779 | |
<I/σ(I)> | 19.16 | 3.78 |
Completeness [%] | 96.7 | 94.6 |
Redundancy | 3.8 | 3.78 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.2 | 10-15% PEG-1000, 50 MM NAKPHOSPHATE PH 6.2 |