4B2F
N-terminal deletion mutant of an outer surface protein BBA73 from Borrelia burgdorferi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-01 |
| Detector | MARRESEARCH MAR 165 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 74.340, 74.340, 95.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.030 - 1.880 |
| R-factor | 0.20606 |
| Rwork | 0.205 |
| R-free | 0.23518 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4axz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.124 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.200 | 1.980 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.060 | 0.490 |
| Number of reflections | 21966 | |
| <I/σ(I)> | 8.5 | 2 |
| Completeness [%] | 98.6 | 99.7 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M MES PH6.5, 18% PEG 3350 |
