4B28
Crystal structure of DMSP lyase RdDddP from Roseobacter denitrificans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 116.850, 116.850, 133.430 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 2.150 |
| R-factor | 0.20851 |
| Rwork | 0.206 |
| R-free | 0.25150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.265 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.420 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.120 | 0.640 |
| Number of reflections | 33321 | |
| <I/σ(I)> | 13.8 | 1.2 |
| Completeness [%] | 90.5 | 45.8 |
| Redundancy | 12.3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 4-4.5 M SODIUM CHLORIDE AND 0.1 M BIS TRIS PH 6.5 |
