4B1R
Tetracycline repressor class D mutant H100A in complex with iso-7- Chlortetracycline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-01 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 68.360, 68.360, 179.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.660 - 1.950 |
| R-factor | 0.20833 |
| Rwork | 0.206 |
| R-free | 0.25976 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2tct |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.668 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0027) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.073 | |
| Number of reflections | 16090 | |
| <I/σ(I)> | 16.6 | 3.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.2 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M MES, PH 6.5, 0.6 M LI2SO4, 16.7 % PEG 4000 |
