4B1L

CARBOHYDRATE BINDING MODULE CBM66 FROM BACILLUS SUBTILIS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-11-17
Detector	RIGAKU R-AXIS IV
Spacegroup name	P 32 2 1
Unit cell lengths	38.616, 38.616, 162.391
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.130 - 1.650
R-factor	0.17998
Rwork	0.176
R-free	0.25591
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4azz
RMSD bond length	0.012
RMSD bond angle	1.456
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.600	1.740
High resolution limit [Å]	1.650	1.650
Rmerge	0.070	0.490
Number of reflections	17659
<I/σ(I)>	18.7	3.1
Completeness [%]	98.7	91.3
Redundancy	8.9	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				25% (W/V) PEG 1500, 100MM MMT BUFFER, 5-6 MM LEVAN BIOSE.