4B1J
Structure of human PARG catalytic domain in complex with ADP-HPD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-17 |
| Detector | RIGAKU SATURN 944 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.848, 90.971, 94.763 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.630 - 2.080 |
| R-factor | 0.17018 |
| Rwork | 0.168 |
| R-free | 0.21475 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a0d |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.416 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.760 | 2.190 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.110 | 0.490 |
| Number of reflections | 32176 | |
| <I/σ(I)> | 12.4 | 2.8 |
| Completeness [%] | 90.2 | 52.7 |
| Redundancy | 6.3 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% PEG 3350, 36.4 MM PCTP-4, 63.6 MM PCTP-10, 0.2 M AMMONIUM SULPHATE |
