4B18
The crystal structure of human Importin alpha 5 with TERT NLS peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 77.15 |
| Detector technology | CCD |
| Collection date | 2012-06-17 |
| Detector | ADSC QUANTUM 4r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.723, 59.916, 69.331 |
| Unit cell angles | 90.00, 96.29, 90.00 |
Refinement procedure
| Resolution | 70.000 - 2.520 |
| R-factor | 0.20101 |
| Rwork | 0.198 |
| R-free | 0.25270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jdq |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.935 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.560 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Rmerge | 0.040 | 0.040 |
| Number of reflections | 21441 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 96.3 | 96.3 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
