4B0N
Crystal structure of PKS-I from the brown algae Ectocarpus siliculosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-23 |
| Detector | ADSC QUANTUM Q4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.320, 83.395, 152.729 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.400 - 2.850 |
| R-factor | 0.21201 |
| Rwork | 0.211 |
| R-free | 0.23587 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ted |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.369 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 3.020 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.100 | 0.330 |
| Number of reflections | 18963 | |
| <I/σ(I)> | 8.5 | 3.4 |
| Completeness [%] | 91.7 | 77.4 |
| Redundancy | 4.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 3 MICROLITER DROPS OF PROTEIN AT 7 MG/ML WERE MIXED WITH 1.5 MICROLITERS OF CRYSTALLIZATION BUFFER (0.1 M BICINE PH 8, 18% PEG 6000) USING THE HANGING DROP VAPOR DIFFUSION METHOD AT 19 DEGREES CELSIUS |
