4B0M
Complex of the Caf1AN usher domain, Caf1M chaperone and Caf1 subunit from Yersinia pestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-26 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.281, 77.436, 95.858 |
| Unit cell angles | 90.00, 98.48, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.186 |
| Rwork | 0.183 |
| R-free | 0.23710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p5v |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.758 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.320 |
| Number of reflections | 47312 | |
| <I/σ(I)> | 17.5 | 2.5 |
| Completeness [%] | 96.9 | 81.5 |
| Redundancy | 3.4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 279 | HANGING DROPS OF 1.5 UL CAF1AN:CAF1M:CAF1 COMPLEX (35 MG/ML) PLUS 1.5 UL OF 15% PEG 3350, 0.1 M NA-CACODYLATE, PH 6.4, 1-2 WEEKS AT 6 DEG C. |
