4B0D

CRYSTAL STRUCTURE OF HEN EGG WHITE LYSOZYME FROM AN AUTO HARVESTED CRYSTAL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	P 43 21 2
Unit cell lengths	78.250, 78.250, 37.915
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.120 - 1.100
R-factor	0.18654
Rwork	0.185
R-free	0.20768
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.026
RMSD bond angle	2.342
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0116)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.000	1.160
High resolution limit [Å]	1.100	1.100
Rmerge	0.090	0.590
Number of reflections	48335
<I/σ(I)>	17.81	2.9
Completeness [%]	100.0	99.9
Redundancy	13.4	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1