4B09
Structure of unphosphorylated BaeR dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-15 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 236.602, 130.401, 198.213 |
| Unit cell angles | 90.00, 90.40, 90.00 |
Refinement procedure
| Resolution | 49.549 - 3.300 |
| R-factor | 0.2262 |
| Rwork | 0.225 |
| R-free | 0.24480 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.055 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.230 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.100 | 0.660 |
| Number of reflections | 89499 | |
| <I/σ(I)> | 7.4 | 1.9 |
| Completeness [%] | 98.8 | 98.6 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.4 | 1.2 M SODIUM ACETATE AND 0.1 M SODIUM CACODYLATE, PH 8.4 |
