4B08
Yeast DNA polymerase alpha, Selenomethionine protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-12 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 73.969, 127.447, 144.076 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.800 - 2.670 |
| R-factor | 0.1972 |
| Rwork | 0.195 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.573 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE: DEV_1078)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.970 | 2.810 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rmerge | 0.130 | 0.660 |
| Number of reflections | 39626 | |
| <I/σ(I)> | 17.9 | 4.6 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 19.7 | 17.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.4 | 0.1 M BICINE PH 8.0-9.4, 6-10% PEG 8000 |
