4B08

Yeast DNA polymerase alpha, Selenomethionine protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-12
Detector	ADSC QUANTUM 315r
Spacegroup name	P 2 21 21
Unit cell lengths	73.969, 127.447, 144.076
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.800 - 2.670
R-factor	0.1972
Rwork	0.195
R-free	0.23400
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.002
RMSD bond angle	0.573
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE: DEV_1078))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.970	2.810
High resolution limit [Å]	2.670	2.670
Rmerge	0.130	0.660
Number of reflections	39626
<I/σ(I)>	17.9	4.6
Completeness [%]	100.0	99.8
Redundancy	19.7	17.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9.4		0.1 M BICINE PH 8.0-9.4, 6-10% PEG 8000