4B04

Crystal structure of the Catalytic Domain of Human DUSP26 (C152S)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	93
Detector technology	CCD
Detector	ADSC QUANTUM 4r
Spacegroup name	P 21 21 21
Unit cell lengths	82.600, 82.780, 91.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.115 - 2.205
R-factor	0.2035
Rwork	0.200
R-free	0.26630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e0t
RMSD bond length	0.022
RMSD bond angle	1.881
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Rmerge	0.060
Number of reflections	31803
<I/σ(I)>	10.1
Completeness [%]	98.3
Redundancy	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1