4AZU

CRYSTAL STRUCTURE ANALYSIS OF OXIDIZED PSEUDOMONAS AERUGINOSA AZURIN AT PH 5.5 AND PH 9.0. A PH-INDUCED CONFORMATIONAL TRANSITION INVOLVES A PEPTIDE BOND FLIP

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	57.650, 80.930, 110.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 1.930 *
R-factor	0.176 *
RMSD bond length	0.011
RMSD bond angle	2.710
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	1.930 *
Rmerge	0.095 *
Total number of observations	148609 *
Number of reflections	34109 *
Completeness [%]	81.3 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	13 (mg/ml)
2	1	drop	ammonium sulphate	1.5 (M)
3	1	drop	lithium nitrate	0.4 (M)
4	1	drop	acetate	0.1 (M)
5	1	reservoir	ammonium sulphate	4.0 (M)
6	1	reservoir	lithium nitrate	0.7 (M)
7	1	reservoir	acetate	0.1 (M)