4AZO
Murine epidermal fatty acid-binding protein (FABP5), apo form, poly- his tag removed
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-14 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 79.320, 79.320, 82.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.330 |
| R-factor | 0.22693 |
| Rwork | 0.224 |
| R-free | 0.27391 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b56 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.402 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.040 | 0.460 |
| Number of reflections | 6932 | |
| <I/σ(I)> | 33.1 | 4 |
| Completeness [%] | 99.2 | 98.9 |
| Redundancy | 10.6 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 200 MM NACL, 50 MM NAAC PH 4.8, 25% PEG3350 AND 5% MPD, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K. CRYPROTECION: 25% GLYCEROL IN MOTHER LIQUOR. |
