4AZN
Murine epidermal fatty acid-binding protein (FABP5), apo form, poly- his tag-mediated crystal packing
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-26 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.290, 64.970, 60.100 |
| Unit cell angles | 90.00, 122.34, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.510 |
| R-factor | 0.21779 |
| Rwork | 0.214 |
| R-free | 0.28186 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b56 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.606 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.050 | 0.220 |
| Number of reflections | 10427 | |
| <I/σ(I)> | 28.4 | 8.2 |
| Completeness [%] | 93.7 | 62 |
| Redundancy | 7.4 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 20% PEG3350, 100 MM BIS-TRIS PH 5.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K. CRYPROTECION: 25% GLYCEROL IN MOTHER LIQUOR. |
