4AZJ
Structural basis of L-phosphoserine binding to Bacillus alcalophilus phosphoserine aminotransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-02-05 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 104.692, 136.194, 151.283 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.876 - 1.500 |
| R-factor | 0.1466 |
| Rwork | 0.146 |
| R-free | 0.16480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w23 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.464 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.060 | 0.330 |
| Number of reflections | 170566 | |
| <I/σ(I)> | 13 | 2.2 |
| Completeness [%] | 99.4 | 99.5 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 1.4 M TRI-SODIUM CITRATE, 0.1 M TRIS-HCL, PH 8.5. C0-CRYSTALLIZATION WITH 25 MM L-PHOSPHOSERINE |
