4AYF
Crystal structure of the complex of the Caf1M:Caf1 chaperone:subunit preassembly complex carrying the Tyr40Ala mutation in the Caf1M chaperone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-10 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.156, 68.966, 65.050 |
| Unit cell angles | 90.00, 94.17, 90.00 |
Refinement procedure
| Resolution | 47.255 - 2.070 |
| R-factor | 0.1951 |
| Rwork | 0.192 |
| R-free | 0.24790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p5v |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.080 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.970 | 2.180 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.070 | 0.350 |
| Number of reflections | 18955 | |
| <I/σ(I)> | 8.27 | 2 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.63 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 4000, TRIS-HCL, PH 7.5, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
