4AY7
methyltransferase from Methanosarcina mazei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.209, 167.419, 45.849 |
| Unit cell angles | 90.00, 117.36, 90.00 |
Refinement procedure
| Resolution | 83.710 - 1.800 |
| R-factor | 0.16556 |
| Rwork | 0.163 |
| R-free | 0.21287 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ay8 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.965 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.710 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.110 | 0.790 |
| Number of reflections | 54605 | |
| <I/σ(I)> | 8.1 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS PH 8.5, 0.2 M MGCL2, 30%(W/W) PEG 4000, 2% 1,1,1,3,3,3-HEXAFLUORO-2-PROPANOL |
