4AXW

CRYSTAL STRUCTURE OF MOUSE CADHERIN-23 EC1-2 AND PROTOCADHERIN-15 EC1- 2, FORM I 2.2A.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-12-21
Detector	E. WESTBROOK NOIR-1 MBC SYSTEM
Spacegroup name	C 1 2 1
Unit cell lengths	173.649, 40.468, 85.187
Unit cell angles	90.00, 102.92, 90.00

Refinement procedure

Resolution	41.080 - 2.230
R-factor	0.17777
Rwork	0.175
R-free	0.23634
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2whv
RMSD bond length	0.017
RMSD bond angle	1.540
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.270
High resolution limit [Å]	2.230	2.230
Rmerge	0.110	0.390
Number of reflections	27442
<I/σ(I)>	10.65	2.69
Completeness [%]	96.2	87.5
Redundancy	3.4	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		0.1 M MES PH 6.5, 15% W/V PEG550MME, 25% GLYCEROL, 0.2M POTASSIUM CHLORIDE