4AXW
CRYSTAL STRUCTURE OF MOUSE CADHERIN-23 EC1-2 AND PROTOCADHERIN-15 EC1- 2, FORM I 2.2A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-21 |
| Detector | E. WESTBROOK NOIR-1 MBC SYSTEM |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.649, 40.468, 85.187 |
| Unit cell angles | 90.00, 102.92, 90.00 |
Refinement procedure
| Resolution | 41.080 - 2.230 |
| R-factor | 0.17777 |
| Rwork | 0.175 |
| R-free | 0.23634 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2whv |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.540 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.270 |
| High resolution limit [Å] | 2.230 | 2.230 |
| Rmerge | 0.110 | 0.390 |
| Number of reflections | 27442 | |
| <I/σ(I)> | 10.65 | 2.69 |
| Completeness [%] | 96.2 | 87.5 |
| Redundancy | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M MES PH 6.5, 15% W/V PEG550MME, 25% GLYCEROL, 0.2M POTASSIUM CHLORIDE |
