4AXT

CRYSTAL STRUCTURE OF HEN EGG WHITE LYSOZYME FROM AN AUTO HARVESTED CRYSTAL, Control Experiment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	P 43 21 2
Unit cell lengths	78.242, 78.242, 37.473
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.660 - 1.100
R-factor	0.1621
Rwork	0.161
R-free	0.17719
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NONE
RMSD bond length	0.023
RMSD bond angle	2.049
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0116)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.480	1.160
High resolution limit [Å]	1.100	1.100
Rmerge	0.070	0.600
Number of reflections	47356
<I/σ(I)>	15.8	3.1
Completeness [%]	99.3	96.7
Redundancy	12.7	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1