4AXS

Structure of Carbamate Kinase from Mycoplasma penetrans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 32 2 1
Unit cell lengths	51.800, 51.800, 174.162
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.860 - 2.500
R-factor	0.2332
Rwork	0.231
R-free	0.28720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e19
RMSD bond length	0.008
RMSD bond angle	1.109
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.860	2.510
High resolution limit [Å]	2.380	2.380
Rmerge	0.070	0.390
Number of reflections	11222
<I/σ(I)>	11.8	2.5
Completeness [%]	97.7	92.2
Redundancy	4.8	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1