4AXO
Structure of the Clostridium difficile EutQ protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2011-07-30 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.332, 66.578, 58.950 |
| Unit cell angles | 90.00, 117.54, 90.00 |
Refinement procedure
| Resolution | 36.503 - 1.000 |
| R-factor | 0.1363 |
| Rwork | 0.136 |
| R-free | 0.14700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pyt |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.601 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.150 | 1.050 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.060 | 0.360 |
| Number of reflections | 148309 | |
| <I/σ(I)> | 11.5 | 2.4 |
| Completeness [%] | 97.9 | 99.4 |
| Redundancy | 2.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | PROTEIN IN 150 MM NACL, 50 MM TRIS.HCL PH 8.0 AT 8 MG/ML. HANGING DROP VAPOUR DIFFUSION, 1/1 UL DROPS OVER 1ML OF PRECIPITANT: 100 MM SODIUM ACETATE PH 4.5, 35 % (W/V) PEG 6000, 200 MM MGCL2. CRYPROTECTED WITH WELL SOLUTION SUPPLEMENTED WITH 20 % (V/V) PEG 300 |
