4AXJ
Structure of the Clostridium difficile EutM protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-01-25 |
| Detector | ADSC CCD |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 69.368, 46.380, 76.400 |
| Unit cell angles | 90.00, 91.56, 90.00 |
Refinement procedure
| Resolution | 23.691 - 1.620 |
| R-factor | 0.1662 |
| Rwork | 0.165 |
| R-free | 0.19590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ewh |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.547 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.200 | 1.710 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.060 | 0.360 |
| Number of reflections | 30458 | |
| <I/σ(I)> | 12 | 3.5 |
| Completeness [%] | 98.3 | 97.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | PROTEIN IN H2O AT 10 MG/ML. CRYSTALLISED BY SITTING DROP VAPOUR DIFFUSION, 100/100 NL DROPS, 100 UL WELL. WELL SOLUTION OF 200 MM LI2SO4, 100 MM PHOSPHATE/CITRATE PH 4.2, 20 % W/V PEG 1000. |
