4AWL
The NF-Y transcription factor is structurally and functionally a sequence specific histone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9,1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.700, 62.560, 139.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.460 - 3.080 |
| R-factor | 0.19418 |
| Rwork | 0.191 |
| R-free | 0.24971 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n1j |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.751 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.560 | 3.250 |
| High resolution limit [Å] | 3.080 | 3.080 |
| Rmerge | 0.060 | 0.170 |
| Number of reflections | 8907 | |
| <I/σ(I)> | 17.4 | 4.4 |
| Completeness [%] | 94.9 | 76.9 |
| Redundancy | 5.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | VAPOUR DIFFUSION METHOD , HANGING DROP, 20-24% PEG3350, 100 MM MES OR CACODYLATE BUFFER, PH 6.5, 50 MM NAF. T ROOM. |
