4AWE
The Crystal Structure of Chrysonilia sitophila endo-beta-D-1,4- mannanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-14 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.360, 78.940, 83.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.930 - 1.400 |
| R-factor | 0.16257 |
| Rwork | 0.161 |
| R-free | 0.20215 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qno |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.237 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0098) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.930 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.120 | 0.390 |
| Number of reflections | 75187 | |
| <I/σ(I)> | 6.5 | 2.2 |
| Completeness [%] | 99.5 | 96 |
| Redundancy | 4.6 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M MAGNESIUM CHLORIDE, 0.1M BIS-TRIS PH 5.5, 25% (W/V) PEG3350 |
