4AW8
X-ray structure of ZinT from Salmonella enterica in complex with zinc ion and PEG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-29 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 58.540, 58.540, 290.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.700 - 2.000 |
| R-factor | 0.21475 |
| Rwork | 0.212 |
| R-free | 0.25696 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 10EK |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.241 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.700 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.100 | 0.237 |
| Number of reflections | 21245 | |
| <I/σ(I)> | 16.3 | 8.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | AMMONIUM SULPHATE 0.2M, SODIUM ACETATE 0.1M PH 4.6, PEG 4000 25%-30% |
