4AVT

Structure of CPHPC bound to Serum Amyloid P Component

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 43 21 2
Unit cell lengths	230.864, 230.864, 281.393
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.942 - 3.200
R-factor	0.189
Rwork	0.189
R-free	0.19740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sac
RMSD bond length	0.015
RMSD bond angle	1.186
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.920	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.090	0.650
Number of reflections	122013
<I/σ(I)>	11.8	2
Completeness [%]	98.0	95.8
Redundancy	3.9	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.6		60MM TRIS-HCL, 80MM NACL, 15% PEG550 MME, pH 7.6