4AVM
Crystal structure of the N-BAR domain of human bridging integrator 2.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-29 |
| Detector | MARRESEARCH MARMOSAIC 300 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 37.886, 81.248, 150.384 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.620 - 1.910 |
| R-factor | 0.1646 |
| Rwork | 0.163 |
| R-free | 0.19820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fic |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.800 |
| Data reduction software | autoPROC |
| Data scaling software | AP_SCALE |
| Phasing software | PHASER (MR) |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 150.380 | 2.020 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.090 | 0.390 |
| Number of reflections | 18417 | |
| <I/σ(I)> | 20.5 | 5.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.5 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 30% JEFFAMINE 2001, 0.1M HEPES PH 7.0, 100 MM NAI. |
