4AV9
Kluyveromyces lactis Hsv2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 159.030, 159.030, 159.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.226 - 3.001 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.24330 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.114 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.050 | 0.550 |
| Number of reflections | 14269 | |
| <I/σ(I)> | 28.3 | 3.9 |
| Completeness [%] | 99.6 | 98.3 |
| Redundancy | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.6 M MGSO4, 0.1 M MES PH6.5 |
