4AV8

Kluyveromyces lactis Hsv2 complete loop 6CD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 41 3 2
Unit cell lengths	156.360, 156.360, 156.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.090 - 3.350
R-factor	0.2021
Rwork	0.200
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4av9
RMSD bond length	0.004
RMSD bond angle	1.145
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.450
High resolution limit [Å]	3.350	3.350
Rmerge	0.060	0.480
Number of reflections	9859
<I/σ(I)>	27.5	5.1
Completeness [%]	99.5	100
Redundancy	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		1.6 M MGSO4, 0.1 M MES PH 6.5