4AU4
Crystal Structure of Hsp47
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-03 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 178.780, 115.110, 188.275 |
| Unit cell angles | 90.00, 107.69, 90.00 |
Refinement procedure
| Resolution | 48.170 - 2.970 |
| R-factor | 0.1825 |
| Rwork | 0.180 |
| R-free | 0.22010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.150 |
| High resolution limit [Å] | 2.970 | 2.970 |
| Rmerge | 0.090 | 0.450 |
| Number of reflections | 73293 | |
| <I/σ(I)> | 11.02 | 2.07 |
| Completeness [%] | 97.4 | 95.9 |
| Redundancy | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
