4ATZ
Ad5 knob in complex with a designed ankyrin repeat protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 109.600, 112.100, 129.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.570 - 1.950 |
| R-factor | 0.16305 |
| Rwork | 0.161 |
| R-free | 0.19333 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1knb |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.350 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.050 | 0.840 |
| Number of reflections | 110875 | |
| <I/σ(I)> | 23 | 2.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.1 | 9.05 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.4 | pH 5.4 |
