4ATL
Crystal structure of Raucaffricine glucosidase in complex with Glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-23 |
| Detector | MARRESEARCH SX-165 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 102.774, 127.329, 215.837 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.900 - 2.520 |
| R-factor | 0.1743 |
| Rwork | 0.173 |
| R-free | 0.21913 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jf6 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.597 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 2.570 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.120 | 0.630 |
| Number of reflections | 46174 | |
| <I/σ(I)> | 17.5 | 3.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.6 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.3M AMMONIUM SULFATE, 0.1M SODIUM ACETATE PH 4.6 BUFFER AND 11% PEG 4000 |
