4AT9
Crystal structure of the NF90-NF45 dimerisation domain complex with UTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-30 |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 83.849, 133.516, 177.983 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.504 - 2.803 |
| R-factor | 0.2372 |
| Rwork | 0.236 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4at7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.999 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.130 | 0.950 |
| Number of reflections | 23950 | |
| <I/σ(I)> | 10.1 | 2.4 |
| Completeness [%] | 96.7 | 95.4 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 12-14% PEG 3350, 200 MM MGCL2, 100 MM MES PH 6.5 AND 5% GLYCEROL |
