4ASS

TubR bound to tubC - 26 bp - from Bacillus thuringiensis serovar israelensis pBtoxis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-05-29
Detector	DECTRIS PILATUS 6M
Spacegroup name	I 2 2 2
Unit cell lengths	87.561, 162.352, 285.394
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.810 - 7.000
R-factor	0.4117
Rwork	0.408
R-free	0.48390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3m9a
RMSD bond length	0.015
RMSD bond angle	1.376
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	142.700	7.060
High resolution limit [Å]	6.700	6.700
Rmerge	0.110	1.586
Number of reflections	3906
<I/σ(I)>	14.2	2
Completeness [%]	99.8	99.8
Redundancy	12.4	13.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		BT TUBR-TUBC CRYSTALS WERE PRODUCED IN 500 NL TO 500 NL PROTEIN TO PRECIPITANT DROPS: 10 MG/ML BT TUBR, 500 UM TUBC-26, 100 MM NA-HEPES PH 7.5, 5 MM MGCL2, 25 % (W/V) POLYACRYLIC ACID 5100 SODIUM SALT.