4ASO
TubR bound to 24 bp of tubC from Bacillus thuringiensis serovar israelensis pBtoxis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-05-29 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 519.251, 63.657, 167.263 |
| Unit cell angles | 90.00, 96.66, 90.00 |
Refinement procedure
| Resolution | 64.469 - 7.000 |
| R-factor | 0.3456 |
| Rwork | 0.344 |
| R-free | 0.37120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m9a |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.449 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 257.870 | 7.380 |
| High resolution limit [Å] | 7.000 | 7.000 |
| Rmerge | 0.090 | 1.642 |
| Number of reflections | 8779 | |
| <I/σ(I)> | 10.9 | 1.3 |
| Completeness [%] | 98.1 | 98.5 |
| Redundancy | 5.9 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | BT TUBR-TUBC CRYSTALS WERE PRODUCED IN 500 NL TO 500 NL PROTEIN TO PRECIPITANT DROPS: 10 MG/ML BT TUBR, 500 UM TUBC-24, 100 MM NA-HEPES PH 7.5, 5 MM MGCL2, 25 % (W/V) POLYACRYLIC ACID 5100 SODIUM SALT. |
