4ASN
TubR from Bacillus megaterium pBM400
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-03-14 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 179.845, 179.845, 114.342 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.085 - 3.500 |
| R-factor | 0.1879 |
| Rwork | 0.186 |
| R-free | 0.22840 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.324 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXCDE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.090 | 3.690 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.050 | 0.430 |
| Number of reflections | 17357 | |
| <I/σ(I)> | 23.1 | 4.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.9 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | BM TUBR CRYSTALS WERE PRODUCED IN 500 NL TO 500 NL PROTEIN TO PRECIPITANT SITTING DROPS: 20 MG/ML BM TUBR, 100 MM TRIS-CL PH 8.5, 0.2 M SODIUM CITRATE, 15 % (V/V) PEG 400. |
