4ASM
Crystal structure of the catalytic domain of beta-agarase D from Zobellia galactanivorans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-03 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.247, 77.268, 83.695 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.800 - 1.500 |
| R-factor | 0.16354 |
| Rwork | 0.162 |
| R-free | 0.18987 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o4z |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.441 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.250 |
| Number of reflections | 55977 | |
| <I/σ(I)> | 21.3 | 9.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.9 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 28-34 % PEG 8000 AND 0,1 M IMIDAZOLE PH 8, 4% ISOPROPANOL |
