4ASC
Crystal structure of the Kelch domain of human KBTBD5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.330, 64.950, 89.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.510 - 1.780 |
| R-factor | 0.18053 |
| Rwork | 0.178 |
| R-free | 0.22623 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2woz |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.661 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.890 | 1.820 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.070 | 0.560 |
| Number of reflections | 34642 | |
| <I/σ(I)> | 13.9 | 3.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.2 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M CITRATE PH 5.3, 20%(W/V) PEG 6000 |
