4AS5
Structure of mouse inositol monophosphatase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-23 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.785, 81.490, 97.139 |
| Unit cell angles | 90.00, 113.77, 90.00 |
Refinement procedure
| Resolution | 43.340 - 2.430 |
| R-factor | 0.1629 |
| Rwork | 0.160 |
| R-free | 0.21440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hhm |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.240 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.340 | 2.560 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.090 | 0.500 |
| Number of reflections | 46562 | |
| <I/σ(I)> | 16.4 | 3.2 |
| Completeness [%] | 98.8 | 96.7 |
| Redundancy | 6.3 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M NAI, 20% W/V PEG 3350, 0.1 M BIS TRIS PROPANE AT PH 7.5 |
