4AR7
X-ray structure of the cyan fluorescent protein mTurquoise
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-06-12 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.452, 62.558, 69.543 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.510 - 1.230 |
R-factor | 0.14383 |
Rwork | 0.143 |
R-free | 0.16639 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ye0 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.923 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.500 | 1.300 |
High resolution limit [Å] | 1.230 | 1.230 |
Rmerge | 0.050 | 0.450 |
Number of reflections | 62924 | |
<I/σ(I)> | 13.5 | 2 |
Completeness [%] | 95.9 | 90.3 |
Redundancy | 4.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 6 MG/ML PROTEIN, 15% PEG8000, 100 MM MGCL2, 100 MM HEPES PH 6.50 |