4AR2
Dodecahedron formed of penton base protein from adenovirus Ad3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 346.040, 348.550, 372.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 258.200 - 3.800 |
| R-factor | 0.28043 |
| Rwork | 0.280 |
| R-free | 0.27809 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aqq |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0114) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.900 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.170 | 0.710 |
| Number of reflections | 249444 | |
| <I/σ(I)> | 4.67 | 1.29 |
| Completeness [%] | 56.8 | 42.1 |
| Redundancy | 2.36 | 1.29 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | HANGING DROP METHOD. RESERVOIR 10-15 % PEG 8000, 100 MM HEPES PH 6.5, 100 MM CACL2 |
